Mechref, Y; Ma, W; Hao, G; Novotny, MV*

Biochemical and Biophysical Research Communications [Biochem. Biophys. Res. Commun.], vol. 255, no. 2, pp. 451-455, 16 Feb 1999

Vomeromodulin, a putative pheromone transporter of the rat vomeronasal organ, was isolated by lectin chromatography, purified, and subjected to a mass spectrometric (MS) system of glycan structural determination. Through a combination of exoglycosidase treatments and measurements by matrix-assisted laser desorption/ionization MS, the N-glycans of vomeromodulin were identified as mainly sialylated and fucosylated biantennary structures. The microheterogeneity of N-glycan structures was also due to the presence of galactose residues with different types of linkages.